Improvement of immunodetection of the transcription factor C/EBP homologous protein by western blot

Accumulation of misfolded proteins within the endoplasmic reticulum (ER) induces an unfolded protein response (UPR) that either restores homeostasis or triggers apoptosis in case of adaptation failure. The three activated branches of UPR lead to IRE1-, PERK- and ATF6- dependent transcriptional induction of the gene encoding the transcription factor C/EBP homologous protein (CHOP) which plays an important role in apoptosis induction. In conventional immunoblotting conditions, detection of CHOP is a difficult task. Using a fixation step, we have optimized the detection of CHOP and this method provides a valuable tool to decipher CHOP involvement in UPR.

Keywords

CHOP Unfolded protein response Western blot Immunodetection Nitrocellulose

Domains

Microbiology and Parasitology

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S0003269720303079.pdf (1009.98 Ko)

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https://hal.univ-reunion.fr/hal-03120150

Submitted on : Friday, June 3, 2022-3:59:15 PM

Last modification on : Wednesday, July 12, 2023-5:07:56 AM

Long-term archiving on: Sunday, September 4, 2022-7:32:00 PM

Dates and versions

hal-03120150 , version 1 (03-06-2022)

Licence

Attribution - NonCommercial

Identifiers

HAL Id : hal-03120150 , version 1
DOI : 10.1016/j.ab.2020.113775
PII : S0003-2697(20)30307-9

Cite

Jonathan Turpin, Étienne Frumence, Daed El Safadi, Olivier Meilhac, Pascale Krejbich-Trotot, et al.. Improvement of immunodetection of the transcription factor C/EBP homologous protein by western blot. Analytical Biochemistry, 2020, 601, pp.113775. ⟨10.1016/j.ab.2020.113775⟩. ⟨hal-03120150⟩

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