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Improvement of immunodetection of the transcription factor C/EBP homologous protein by western blot

Jonathan Turpin 1 Etienne Frumence 1 Daed El Safadi 1 Olivier Meilhac 2, 3 Pascale Krejbich-Trotot 1 Wildriss Viranaicken 1
1 PIMIT - Processus Infectieux en Milieu Insulaire Tropical
2 DéTROI - Diabète athérothrombose et thérapies Réunion Océan Indien
3 CHU La Réunion - Centre Hospitalier Universitaire de La Réunion
Abstract : Accumulation of misfolded proteins within the endoplasmic reticulum (ER) induces an unfolded protein response (UPR) that either restores homeostasis or triggers apoptosis in case of adaptation failure. The three activated branches of UPR lead to IRE1-, PERK- and ATF6- dependent transcriptional induction of the gene encoding the transcription factor C/EBP homologous protein (CHOP) which plays an important role in apoptosis induction. In conventional immunoblotting conditions, detection of CHOP is a difficult task. Using a fixation step, we have optimized the detection of CHOP and this method provides a valuable tool to decipher CHOP involvement in UPR.
Keywords : CHOP Unfolded protein response Western blot Immunodetection Nitrocellulose
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https://hal.univ-reunion.fr/hal-03120150
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Submitted on : Monday, January 25, 2021 - 12:43:27 PM
Last modification on : Wednesday, June 30, 2021 - 9:40:15 PM

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UNIV-REUNION | AFRIQ | PIMIT | DETROI | CNRS | PAPANGUE

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Jonathan Turpin, Etienne Frumence, Daed El Safadi, Olivier Meilhac, Pascale Krejbich-Trotot, et al.. Improvement of immunodetection of the transcription factor C/EBP homologous protein by western blot. Analytical Biochemistry, Elsevier Masson, 2020, 601, pp.113775. ⟨10.1016/j.ab.2020.113775⟩. ⟨hal-03120150⟩

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