Improvement of immunodetection of the transcription factor C/EBP homologous protein by western blot

Accumulation of misfolded proteins within the endoplasmic reticulum (ER) induces an unfolded protein response (UPR) that either restores homeostasis or triggers apoptosis in case of adaptation failure. The three activated branches of UPR lead to IRE1-, PERK- and ATF6- dependent transcriptional induction of the gene encoding the transcription factor C/EBP homologous protein (CHOP) which plays an important role in apoptosis induction. In conventional immunoblotting conditions, detection of CHOP is a difficult task. Using a fixation step, we have optimized the detection of CHOP and this method provides a valuable tool to decipher CHOP involvement in UPR.

Mots clés

CHOP Unfolded protein response Western blot Immunodetection Nitrocellulose

Domaines

Microbiologie et Parasitologie

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S0003269720303079.pdf (1009.98 Ko)

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https://hal.univ-reunion.fr/hal-03120150

Soumis le : vendredi 3 juin 2022-15:59:15

Dernière modification le : vendredi 27 septembre 2024-09:14:19

Archivage à long terme le : dimanche 4 septembre 2022-19:32:00

Dates et versions

hal-03120150 , version 1 (03-06-2022)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

HAL Id : hal-03120150 , version 1
DOI : 10.1016/j.ab.2020.113775
PII : S0003-2697(20)30307-9

Citer

Jonathan Turpin, Étienne Frumence, Daed El Safadi, Olivier Meilhac, Pascale Krejbich-Trotot, et al.. Improvement of immunodetection of the transcription factor C/EBP homologous protein by western blot. Analytical Biochemistry, 2020, 601, pp.113775. ⟨10.1016/j.ab.2020.113775⟩. ⟨hal-03120150⟩

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