A web-based tool for rational screening of mutants libraries using ProSAR

Magali Berland; Bernard Offmann; Isabelle Andre; Magali Remaud-Simeon; Philippe Charton

A web-based tool for rational screening of mutants libraries using ProSAR

Magali Berland ¹ Bernard Offmann ¹ Isabelle Andre ² Magali Remaud-Simeon ² Philippe Charton ¹

1 DSIMB Réunion - Dynamique des Structures et Interactions des Macromolécules Biologiques- Pôle de La Réunion

DSIMB - Dynamique des Structures et Interactions des Macromolécules Biologiques, UR - Université de La Réunion : UMR_S665

2 LISBP - Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés

Abstract : In directed evolution experiments, it is at stake to have methods to screen efficiently the mutant libraries. We propose a web-based tool that implements an established in silico method for the rational screening of mutant libraries. The method, known as ProSAR, attempts to link sequence data to activity. The method uses statistical models trained on small experimental datasets provided by the user. These can integrate potential epistatic interactions between mutations and be used in many diverse biological contexts. It drastically improves the search for leading mutants. The tool is freely available to non-commercial users at http://bo-protscience.fr/prosar/.

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Quantitative Methods [q-bio.QM]

Complete list of metadatas

Cited literature [13 references] Display Hide Download

http://hal.univ-reunion.fr/hal-01471908
Contributor : Philippe Charton <>
Submitted on : Wednesday, May 22, 2019 - 3:32:46 PM
Last modification on : Monday, September 2, 2019 - 9:42:58 AM

A web-based tool for rational screening of mutants libraries using ProSAR

File

Identifiers

Collections

Citation

Export

Metrics

263

44

Contact

A web-based tool for rational screening of mutants libraries using ProSAR

File

Identifiers

Collections

Citation

Export

Share

Metrics

263

44

Contact