In Vitro Analyses of the Multifocal Effects of Natural Alkaloids Berberine, Matrine, and Tabersonine against the O’nyong-nyong Arthritogenic Alphavirus Infection and Inflammation - Université de La Réunion Access content directly
Journal Articles Pharmaceuticals Year : 2023

In Vitro Analyses of the Multifocal Effects of Natural Alkaloids Berberine, Matrine, and Tabersonine against the O’nyong-nyong Arthritogenic Alphavirus Infection and Inflammation

Abstract

O'nyong-nyong virus (ONNV) is a member of the reemerging arthritogenic alphaviruses that cause chronic debilitating polyarthralgia and/or polyarthritis via their tropism for the musculoskeletal system. Thus, the discovery of dual antiviral and anti-inflammatory drugs is a great challenge in this field. We investigated the effects of the common plant-derived alkaloids berberine (isoquinoline), matrine (quinolizidine), and tabersonine (indole) at a non-toxic concentration (10 µM) on a human fibroblast cell line (HS633T) infected by ONNV (MOI 1). Using qRT-PCR analyses, we measured the RNA levels of the gene coding for the viral proteins and for the host cell immune factors. These alkaloids demonstrated multifocal effects by the inhibition of viral replication, as well as the regulation of the type-I interferon antiviral signaling pathway and the inflammatory mediators and pathways. Berberine and tabersonine proved to be the more valuable compounds. The results supported the proposal that these common alkaloids may be useful scaffolds for drug discovery against arthritogenic alphavirus infection.
Fichier principal
Vignette du fichier
pharmaceuticals-16-01125.pdf (3.77 Mo) Télécharger le fichier
Origin Files produced by the author(s)
Licence

Dates and versions

hal-04458774 , version 1 (15-02-2024)

Licence

Identifiers

Cite

Anne-Laure Sandenon Seteyen, Pascale Guiraud, Philippe Gasque, Emmanuelle Girard-Valenciennes, Jimmy Sélambarom. In Vitro Analyses of the Multifocal Effects of Natural Alkaloids Berberine, Matrine, and Tabersonine against the O’nyong-nyong Arthritogenic Alphavirus Infection and Inflammation. Pharmaceuticals, 2023, 16 (8), pp.1125. ⟨10.3390/ph16081125⟩. ⟨hal-04458774⟩
7 View
4 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More