The austral spring in the Southern Hemisphere presents temporary reductions in ozone content mainly in the Antarctic region known as the Antarctic Ozone Hole (AOH). However, studies show an influence in mid-latitude regions, such as southern Brazil, where days with temporary decreases in the total column ozone (TCO) are identified. The main objective of this work is to investigate this influence of AOH on the southern region of Brazil, using data from the TCO and vertical profiles that will help to identify the preferential height at which these decreases occur in southern Brazil, in addition to analyzing the atmospheric dynamic behavior during these events in the period 42 years of data (1979 to 2020). The methodology used comprises the analysis of average daily data of the total column of ozone through ground-based instruments (Brewer Spectrophotometer), satellite data (TOMS and OMI), and to compare reanalysis data from the ECMWF-ERA5, for the identification of events of influence of the AOH on the southern region of Brazil. The analysis of the vertical content of ozone (O3) data from the TIMED/SABER satellite provides daily data from 15 to 110 km in height and has 19 years of O3 profiles available in the period from 2002 to 2020. From this, 102 events were identified that influenced Santa Maria (29.4º S; 53.7º W), in the south of Brazil, with a temporary decrease in the ozone content in the period, where between 24.1–28 km of altitude the more significant reductions in O3 during events. In the dynamic analysis, the stratospheric fields showed an increase in the absolute potential vorticity, mainly in September and October. The conceptual models in the horizontal and vertical section of the atmosphere explain the action of the stratospheric and tropospheric jet during the occurrence of events of decrease in the O3 content in Santa Maria. It was possible to identify the strong influence on the development of these events through the connection of the stratospheric jet (polar vortex) with the tropospheric jets (polar and subtropical jet) at medium and high levels of the atmosphere.