Enhanced oxidative stress in adipose tissue from diabetic mice, possible contribution of glycated albumin

Florence Boyer; Nicolas Diotel; Dorothée Girard; Philippe Rondeau; M. Faadiel Essop; Emmanuel Bourdon

Enhanced oxidative stress in adipose tissue from diabetic mice, possible contribution of glycated albumin

Florence Boyer ^{1, 2} Nicolas Diotel ^{1, 2} Dorothée Girard ^{1, 2} Philippe Rondeau ^{1, 2} M. Faadiel Essop ³ Emmanuel Bourdon ^{1, 2}

1 DéTROI - Diabète athérothrombose et thérapies Réunion Océan Indien

2 CYROI - Cyclotron Réunion Océan Indien

3 Stellenbosch University

Abstract : Although enhanced oxidative stress and proteotoxicity constitute major contributors to the pathogenesis of multiple diseases, there is limited understanding of its role in adipose tissue. Here, we aimed at evaluating oxidative stress biomarkers in adipocytes from diabetic/obese db/db mice. The current study revealed that reactive oxygen species production was upregulated in adipocytes, together with lipid peroxidation 4-hydroxynonenal accumulation, and altered proteolytic and antioxidant activities. In parallel, acute exposure of 3T3L1 adipocyte cell lines to glycated albumin (known to be enhanced with diabetes) also elicited intracellular free radical formation. Our data provide novel insights into redox and proteolytic homeostasis in adipocytes.

Keywords : Male Mice Immunohistochemistry Animals Antioxidants Biomarkers Cell Line Cell Survival Diabetes Diabetes Mellitus Experimental Free Radicals Glycated albumin Homozygote Hyperglycemia Adipose Tissue Adipocytes 3T3-L1 Cells Inbred C57BL Oxidative Stress Proteasome Reactive Oxygen Species Serum Albumin Sulfhydryl Compounds

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Human health and pathology / Endocrinology and metabolism

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