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Journal articles
Evaluation of Direct Wet Transesterification Methods on Yeast and Fungal Biomass Grown on Sugarcane Distillery Spent Wash
Abstract : The present work deals with three methods proposed for the direct transesterification of lipids in the filamentous fungi Aspergillus niger and in the oleaginous yeast Yarrowia lipolytica, grown on distillery spent wash (DSW). For comparison, the reported amount of fatty acid methyl esters (FAMEs) generated by the improved methods applied to dry and wet biomass was normalized against the amount of FAMEs yielded via a two-step extraction/methanolysis method from the same samples. To increase the esterification reactions yield from wet biomass, modifications of the process were evaluated, including: the use of a co-solvent; hydrolysis pretreatment; and the use of a double catalyst. The methylated derivatives generated by each method were then analyzed by gas chromatography with flame ionization detector (GC-FID). It is shown that direct transesterification yielded higher amounts of FAMEs than the two-step methods in both microorganisms, with normalized FAMEs productions of: 146 % (A. niger) and 190 % (Y. lipolytica) from dry biomass, and 119 % (A. niger) and 143 % (Y. lipolytica) from wet biomass, respectively. In the case of wet Y. lipolytica, the double catalyst improvement approach generated 20 % higher FAMEs production than the direct acid methanolysis of the same material. The contributions of the methods parameters for improved esterification and for the FAMEs production profile for Y. lipolytica are also discussed.
Keywords :
Direct methanolysis
ALnAME: Α-Linolenic Acid Methyl Ester
DSW: Distillery Spent Wash
FAME: Fatty Acid Methyl Ester
LAME: Linoleic Acid Methyl Ester
MUFA: Monounsaturated Fatty Acids
OAME: Oleic Acid Methyl Ester
PAME: Palmitic Acid Methyl Ester
Poame: Palmitoleic Acid Methyl Ester
PUFA: Polyunsaturated Fatty Acids
SFA: Saturated Fatty Acids
SCO: Single Cell Oil SAME: Stearic Acid Methyl Ester
UAM: Ultrasound Assisted Maceration
Oleaginous Fungi
Double catalys
Hydrolysis
Co-Solvent
Distillery waste water
ASE: Accelerated Solvent Extraction
Cited literature [41 references]
https://hal.univ-reunion.fr/hal-01475073
Contributor : Réunion Univ <>
Submitted on : Thursday, February 23, 2017 - 2:30:11 PM
Last modification on : Wednesday, July 1, 2020 - 12:39:22 PM
Long-term archiving on: : Wednesday, May 24, 2017 - 1:55:52 PM
Contributor : Réunion Univ <>
Submitted on : Thursday, February 23, 2017 - 2:30:11 PM
Last modification on : Wednesday, July 1, 2020 - 12:39:22 PM
Long-term archiving on: : Wednesday, May 24, 2017 - 1:55:52 PM
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