The temperature retrieved from the Sounding of the Atmosphere using Broadband Emission Radiometry (SABER) onboard Thermosphere Ionosphere Mesosphere Energetics and Dynamics (TIMED) satellite during January 2002 to September 2015 are used in this study to delineate the differences of middle atmospheric thermal structure in the Northern Hemisphere (NH) and Southern Hemisphere (SH). Two stations namely Mt. Abu (24.59°N, 72.70°E) in NH and Reunion Island (21.11°S, 55.53°E) in SH are chosen over sub-tropical regions. Temperature climatology from SABER observations suggests that stratopause is warmer, and upper mesosphere is cooler in NH as compared to SH. Three atmospheric models are used to understand the monthly thermal structure differences for different altitudes. Moreover, semi-annual, annual and quasi-biennial oscillations are studied using Lomb Scargle Periodogram and Wavelet transform techniques. Over NH, summer and winter season are warmer (~4 K) and cooler (~3 K) respectively in stratosphere as compared to SH. It is important to note here that Mt. Abu temperature is warmer (~9 K) than Reunion Island in winter but in summer season Mt. Abu temperature is cooler in upper mesosphere and above mesosphere NH shows warming. Results show that annual oscillations are dominated in both hemisphere as compared to semi-annual and quasi-biennial oscillations. In upper mesosphere, strength of annual oscillations is substantial in NH, while semi-annual oscillations are stronger in SH. Wavelet analyses found that annual oscillations are significant in NH near mesopause, while semi-annual oscillations are strengthening in SH.