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CD93/AA4.1: A Novel Regulator of Inflammation in Murine Focal Cerebral Ischemia

Abstract : The stem-cell marker CD93 (AA4.1/C1qRp) has been described as a potential complement C1q-receptor. Its exact molecular function, however, remains unknown. By using global expression profiling we showed that CD93-mRNA is highly induced after transient focal cerebral ischemia. CD93 protein is upregulated in endothelial cells, but also in selected macrophages and microglia. To elucidate the potential functional role of CD93 in postischemic brain damage, we used mice with a targeted deletion of the CD93 gene. After 30 min of occlusion of the middle cerebral artery and 3 d of reperfusion these mice displayed increased leukocyte infiltration into the brain, increased edema, and significantly larger infarct volumes (60.8 ± 52.2 versus 23.9 ± 16.6 mm3) when compared with wild-type (WT) mice. When the MCA was occluded for 60 min, after 2 d of reperfusion the CD93 knockout mice still showed more leukocytes in the brain, but the infarct volumes were not different from those seen in WT animals. To further explore CD93-dependent signaling pathways, we determined global transcription profiles and compared CD93-deficient and WT mice at various time points after induction of focal cerebral ischemia. We found a highly significant upregulation of the chemokine CCL21/Exodus-2 in untreated and treated CD93-deficient mice at all time points. Induction of CCL21 mRNA and protein was confirmed by PCR and immunohistochemistry. CCL21, which was formerly shown to be released by damaged neurons and to activate microglia, contributes to neurodegeneration. Thus, we speculate that CD93-neuroprotection is mediated via suppression of the neuroinflammatory response through downregulation of CCL21.
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Submitted on : Tuesday, February 16, 2016 - 8:43:08 AM
Last modification on : Sunday, June 26, 2022 - 12:53:52 AM

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Denise Harhausen, Vincent Prinz, Gina Ziegler, Karen Gertz, Matthias Endres, et al.. CD93/AA4.1: A Novel Regulator of Inflammation in Murine Focal Cerebral Ischemia. Journal of Immunology, 2010, 184 (11), pp.6407--6417. ⟨10.4049/jimmunol.0902342⟩. ⟨hal-01274623⟩



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