First molecular detection of Rickettsia africae in ticks from the Union of the Comoros - Archive ouverte HAL Access content directly
Journal Articles Parasites & Vectors Year : 2014

First molecular detection of Rickettsia africae in ticks from the Union of the Comoros

(1) , (1) , (1) , (1) , (2) , (2) , (1)
1
2

Abstract

BACKGROUND: Rickettsia africae is the agent of African tick bite fever, a disease transmitted by ticks in sub-Saharan Africa. In Union of the Comoros, a recent study reported the presence of a Rickettsia africae vector but no information has been provided on the circulation of the pathogenic agent in this country. METHODS: To evaluate the possible circulation of Rickettsia spp. in Comorian cattle, genomic DNA was extracted from 512 ticks collected either in the Union of the Comoros or from animals imported from Tanzania and subsequently tested for Rickettsia infection by quantitative PCR. RESULTS: Rickettsia africae was detected in 90% (60/67) of Amblyomma variegatum, 1% (1/92) of Rhipicephalus appendiculatus and 2.7% (8/296) of Rhipicephalus (Boophilus) microplus ticks collected in the Union of the Comoros, as well as in 77.14% (27/35) of Amblyomma variegatum ticks collected from imported cattle. Partial sequences of both bacterial gltA and ompA genes were used in a phylogenetic analysis revealing the presence of several haplotypes, all included within the Rickettsia africae clade. CONCLUSIONS: Our study reports the first evidence of Rickettsia africae in ticks collected from the Union of the Comoros. The data show a significant difference of infection rate of Rickettsia africae infected ticks between the Islands, with maximum rates measured in Grande Comore Island, sheltering the main entry port for live animal importation from Tanzania. The high infection levels reported herein indicate the need for an in-depth assessment of the burden of rickettsioses in the Union of the Comoros, especially among those at risk of infection, such as cattle herders.
Fichier principal
Vignette du fichier
First_molecular_detection_Rickettsia_africae_ticks_union_comoros.pdf (618.19 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-01274564 , version 1 (21-06-2018)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Amina Yssouf, Cristina Socolovschi, Tahar Kernif, Sarah Temmam, Erwan Lagadec, et al.. First molecular detection of Rickettsia africae in ticks from the Union of the Comoros. Parasites & Vectors, 2014, 7 (1), pp.444. ⟨10.1186/1756-3305-7-444⟩. ⟨hal-01274564⟩
177 View
114 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More