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Genome sequence of Xanthomonas fuscans subsp. fuscans strain 4834-R reveals that flagellar motility is not a general feature of xanthomonads

Armelle Darrasse 1, 2 Sebastien Carrere 3 Valérie Barbe 4 Tristan Boureau 2, 1 Mario L. Arrieta-Ortiz 5, 1, 3 Sophie Bonneau 1, 2 Martial Briand 1, 2 Christelle Brin 1, 2 Stéphane Cociancich 6 Karine Durand 1, 2 Stéphanie Fouteau 4 Lionel Gagnevin 7 Fabien Guérin 7 Endrick Guy 3 Arnaud Indiana 1, 2 Ralf Koebnik 8, 9 Emmanuelle Lauber 3 Alejandra Munoz Bodnar 5 Laurent D. Noel 3 Isabelle Pieretti 6 Stéphane Poussier 7, 1, 2 Olivier Pruvost 7 Isabelle Robene-Soustrade 7 Philippe Rott 6 Monique Royer 6 Laurana Serres-Giardi 1, 2 Boris Szurek 10, 8 Marie-Anne van Sluys Valérie Verdier 10, 8 Christian Vernière 7 Matthieu Arlat 3, 1, 2 Charles Manceau 2, 1 Marie Agnès Jacques 1, 2
1 IRHS - Institut de Recherche en Horticulture et Semences
2 UNAM - PRES Université Nantes Angers Le Mans
3 LIPM - Laboratoire des interactions plantes micro-organismes
4 GENOSCOPE - Genoscope - Centre national de séquençage [Evry]
5 ULA - Universidad de Los Andes [Venezuela]
6 UMR BGPI - Biologie et Génétique des Interactions Plante-Parasite
7 UMR PVBMT - Peuplements végétaux et bioagresseurs en milieu tropical
8 IRD - Institut de Recherche pour le Développement
9 UMR RPB - Résistance des plantes aux bio-agresseurs
10 Centre IRD de Montpellier
Abstract : Background Xanthomonads are plant-associated bacteria responsible for diseases on economically important crops. Xanthomonas fuscans subsp. fuscans (Xff) is one of the causal agents of common bacterial blight of bean. In this study, the complete genome sequence of strain Xff 4834-R was determined and compared to other Xanthomonas genome sequences. Results Comparative genomics analyses revealed core characteristics shared between Xff 4834-R and other xanthomonads including chemotaxis elements, two-component systems, TonB-dependent transporters, secretion systems (from T1SS to T6SS) and multiple effectors. For instance a repertoire of 29 Type 3 Effectors (T3Es) with two Transcription Activator-Like Effectors was predicted. Mobile elements were associated with major modifications in the genome structure and gene content in comparison to other Xanthomonas genomes. Notably, a deletion of 33 kbp affects flagellum biosynthesis in Xff 4834-R. The presence of a complete flagellar cluster was assessed in a collection of more than 300 strains representing different species and pathovars of Xanthomonas. Five percent of the tested strains presented a deletion in the flagellar cluster and were non-motile. Moreover, half of the Xff strains isolated from the same epidemic than 4834-R was non-motile and this ratio was conserved in the strains colonizing the next bean seed generations. Conclusions This work describes the first genome of a Xanthomonas strain pathogenic on bean and reports the existence of non-motile xanthomonads belonging to different species and pathovars. Isolation of such Xff variants from a natural epidemic may suggest that flagellar motility is not a key function for in planta fitness. (Résumé d'auteur)
Keywords : Seed-borne pathogen Secretion system Insertion sequence Bean Effector Chemotaxis
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INRAE | CNRS | UNIV-ANGERS | PAPANGUE | FST-REUNION | GENOSCOPE | MONTPELLIER-SUPAGRO | IRHS | CEA-DRF | JACOB | INRA | CEA | CIRAD | PVBMT | UNAM | UNIV-REUNION | AGROPOLIS | IRD | INRAEOCCITANIEMONTPELLIER | AFRIQ

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Armelle Darrasse, Sebastien Carrere, Valérie Barbe, Tristan Boureau, Mario L. Arrieta-Ortiz, et al.. Genome sequence of Xanthomonas fuscans subsp. fuscans strain 4834-R reveals that flagellar motility is not a general feature of xanthomonads. BMC Genomics, BioMed Central, 2013, 14, pp.761--790. ⟨10.1186/1471-2164-14-761⟩. ⟨hal-01199326⟩

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