Fatty acids do not pay the toll: effect of SFA and PUFA on human adipose tissue and mature adipocytes inflammation

Abstract : Background On the basis that high fat diet induces inflammation in adipose tissue, we wanted to test the effect of dietary saturated and polysunsaturated fatty acids on human adipose tissue and adipocytes inflammation. Moreover we wanted to determine if TLR2 and TLR4 are involved in this pathway. Methods Human adipose tissue and adipocytes primary cultures were treated with endotoxin-free BSA conjugated with SFA (lauric acid and palmitic acid - LA and PA) and PUFA (eicosapentaeneic acid, docosahexaenoic acid and oleic acid - EPA, DHA and OA) with or without LPS. Cytokines were then assayed by ELISA (TNF-alpha, IL-6 and MCP-1). In order to determine if TLR2 and TLR4 are activated by fatty acid (FA), we used HEK-Blue cells transfected by genes from TLR2 or TLR4 pathways associated with secreted alkaline phosphatase reporter gene. Results None of the FA tested in HEK-Blue cells were able to activate TLR2 or TLR4, which is concordant with the fact that after FA treatment, adipose tissue and adipocytes cytokines levels remain the same as controls. However, all the PUFA tested: DHA, EPA and to a lesser extent OA down-regulated TNF-alpha, IL-6 and MCP-1 secretion in human adipose tissue and adipocytes cultures. Conclusions This study first confirms that FA do not activate TLR2 and TLR4. Moreover by using endotoxin-free BSA, both SFA and PUFA tested were not proinflammatory in human adipose tissue and adipocytes model. More interestingly we showed that some PUFA exert an anti-inflammatory action in human adipose tissue and adipocytes model. These results are important since they clarify the relationship between dietary fatty acids and inflammation linked to obesity.
Document type :
Journal articles
Complete list of metadatas

Cited literature [53 references]  Display  Hide  Download

http://hal.univ-reunion.fr/hal-01198331
Contributor : Nicolas Alarcon <>
Submitted on : Tuesday, June 19, 2018 - 8:46:47 AM
Last modification on : Thursday, March 28, 2019 - 11:24:15 AM
Long-term archiving on : Tuesday, September 25, 2018 - 3:50:45 AM

File

Fatty_acids_do_not_pay_the_tol...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Ravi Kumar Murumalla, Manoj Kumar Gunasekaran, Jibesh Kumar Padhan, Karima Bencharif, Lydie Gence, et al.. Fatty acids do not pay the toll: effect of SFA and PUFA on human adipose tissue and mature adipocytes inflammation. Lipids in Health and Disease, BioMed Central, 2012, 11 (1), pp.1--9. ⟨10.1186/1476-511X-11-175⟩. ⟨hal-01198331⟩

Share

Metrics

Record views

150

Files downloads

97