A web-based tool for rational screening of mutants libraries using ProSAR

Abstract : In directed evolution experiments, it is at stake to have methods to screen efficiently the mutant libraries. We propose a web-based tool that implements an established in silico method for the rational screening of mutant libraries. The method, known as ProSAR, attempts to link sequence data to activity. The method uses statistical models trained on small experimental datasets provided by the user. These can integrate potential epistatic interactions between mutations and be used in many diverse biological contexts. It drastically improves the search for leading mutants. The tool is freely available to non-commercial users at http://bo-protscience.fr/prosar/.
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Protein Engineering, Design and Selection, Oxford University Press (OUP), 2014, 27 (10), pp.375 - 381. 〈10.1093/protein/gzu035〉
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http://hal.univ-reunion.fr/hal-01471908
Contributeur : Philippe Charton <>
Soumis le : lundi 20 février 2017 - 13:21:56
Dernière modification le : vendredi 16 novembre 2018 - 01:37:27

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Magali Berland, Bernard Offmann, Isabelle Andre, Magali Remaud-Simeon, Philippe Charton. A web-based tool for rational screening of mutants libraries using ProSAR. Protein Engineering, Design and Selection, Oxford University Press (OUP), 2014, 27 (10), pp.375 - 381. 〈10.1093/protein/gzu035〉. 〈hal-01471908〉

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