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Potentiel néphroprotecteur des molécules issues de la biodiversité végétale réunionnaise : focus sur Antirhea borbonica

Abstract : Regardless of the initial insult, the progression of most forms of chronic renal disease (CKD) results in tubulointerstitial fibrosis. It is now well admitted that the progression of fibrosis in CKD is closely correlated to the progressive loss of renal function leading to end stage renal disease (ESRD). Interstitial fibrosis is characterized by the progressive accumulation of extracellular matrix (ECM) proteins in the tubulointerstitial compartment. Since decade, in humans, blockade of the renin-angiotensin system with angiotensin-converting enzyme inhibitors and angiotensin type I receptor antagonists remains the only effective therapy to slow down the progression toward ESRD. Therefore, the challenge for nephrologists and scientists is to discover alternative molecules or therapies to slow down, stop and if possible, reverse the progression of renal fibrosis towards ESRD. In Reunion Island, since 2012, 27 medicinal plants have been registered at the French pharmacopeia. Most of them are known for their antioxidant and anti-inflammatory activities related mostly to their polyphenols content suggesting putative antifibrotic effect. The main objective of the present study was to evaluate the hypothetical nephroprotective effects (antioxidant, anti-inflammatory and anti-fibrotic) of polyphenols of the medicinal plant Antirhea borbonica (A.borbonica). In a first set of experiments, by using selective liquid chromatography–tandem mass spectrometry, we performed polyphenol identification and quantification from A.borbonica leaves. Our data show high phenolic acids and flavonoids concentrations. In addition, we performed a zebrafish embryo acute toxicity showing the non-toxicity of the doses used in traditional medicine. In a second set of experiments by using an accelerated mouse model of renal tubulointerstitial fibrosis we provided evidences that the oral administration of a polyphenol-rich extract of A.borbonica induced a preventive as well as curative effect on the progression of renal tubulointerstitial fibrosis. These nephroprotective effects go through the reduction in the three phases of the fibrotic process: (i) macrophage infiltration, (ii) myofibroblast appearance and (iii) extracellular matrix accumulation. Furthermore, by using desorption electrospray ionization-high resolution/mass spectrometry (DESI-HR/MS) imaging were able to visualize, for the first time, CA in the kidney cortex. Taken together our results show the therapeutic antifibrotic potential of polyphenol-rich extract of A.borbonica. Our scientific approach could be used as an example to study the nephroprotective effects of other medicinal plants registered at the French pharmacopeia.
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Submitted on : Monday, October 25, 2021 - 2:05:12 PM
Last modification on : Wednesday, October 27, 2021 - 3:52:40 AM

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  • HAL Id : tel-03401641, version 1

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Bryan Veeren. Potentiel néphroprotecteur des molécules issues de la biodiversité végétale réunionnaise : focus sur Antirhea borbonica. Biologie végétale. Université de la Réunion, 2021. Français. ⟨NNT : 2021LARE0025⟩. ⟨tel-03401641⟩

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