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Tannin phenotyping of the Vitaceae reveals a phylogenetic linkage of epigallocatechin in berries and leaves

Jean-Marc Brillouet 1 Charles Romieu 2 Roberto Bacilieri 2 Peter Nick 3 Anna Trias-Blasi 4 Erika Maul 5 Katalin Solymosi 6 Peter Teszlák 7 Jiang-Fu Jiang 8 Lei Sun 8 Danielle Ortolani 9 Jason P Londo 10 Ben Gutierrez 11 Bernard Prins 12 Marc Reynders 13 Frank van Caekenberghe 14 David Maghradze 15 Cecile Marchal 16 Amir Sultan 17 Jean-Francois Thomas 18 Daniel Scherberich 18 Helene Fulcrand 1 Laurent Roumeas 1 Guillaume Billerach 1 Vugar Salimov 19 Mirza Musayev 20 Muhammad Ejaz Ul Islam Dar 21 Jean-Benoit Peltier 22 Michel Grisoni 23 
Abstract : Abstract Background and Aims Condensed tannins, responsible for berry and wine astringency, may have been selected during grapevine domestication (Narduzzi et al., 2015). This work examines the phylogenetic distribution of condensed tannins throughout the Vitaceae phylogenetic tree. Methods Green berries and mature leaves of representative true-to-type members of the Vitaceae were collected before “véraison”, freeze-dried, pulverised, and condensed tannins measured following depolymerization by nucleophilic addition of 2-mercaptoethanol to the C4 of the flavan-3-ol units in an organic acidic medium. Reaction products were separated and quantitated by UPLC/DAD/MS. Key Results and Conclusions The original ability to incorporate epigallocatechin (EGC) into grapevine condensed tannins was lost independently in both the American and Eurasian/Asian branches of the Vitaceae, with exceptional cases of reversion to the ancestral EGC phenotype. This is particularly true in the genus Vitis, where we now find two radically distinct groups differing with respect to EGC content. While Vitis species from Asia are void of EGC, 50% of the New World Vitis harbour EGC. Interestingly, the presence of EGC is tightly coupled with the degree of leaf margin serration. Noticeably, the rare Asian EGC-forming species are phylogenetically close to Vitis vinifera, the only remnant representative of Vitis in Eurasia. Both the wild ancestral V. vinifera subsp. sylvestris as well as the domesticated V. vinifera subsp. sativa can accumulate EGC and activate galloylation biosynthesis that compete for photoassimilates and reductive power.
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Submitted on : Monday, June 20, 2022 - 11:31:06 AM
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Jean-Marc Brillouet, Charles Romieu, Roberto Bacilieri, Peter Nick, Anna Trias-Blasi, et al.. Tannin phenotyping of the Vitaceae reveals a phylogenetic linkage of epigallocatechin in berries and leaves. Annals of Botany, Oxford University Press (OUP), 2022, ⟨10.1093/aob/mcac077⟩. ⟨hal-03699434⟩



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