Quercetin can reduce viral RNA level of O’nyong-nyong virus and resulting innate immune cytokine responses in cultured human synovial fibroblasts - Université de La Réunion Access content directly
Journal Articles Scientific Reports Year : 2021

Quercetin can reduce viral RNA level of O’nyong-nyong virus and resulting innate immune cytokine responses in cultured human synovial fibroblasts

Abstract

O'nyong-nyong virus is an alphavirus closely related to chikungunya virus, causing arthralgia, rash and fever. Alphaviruses mainly target synovial fibroblasts and persists in the joints of patients, possibly leading to chronic arthritis. To date, no specific antiviral treatment is available for ONNV infection and induced-inflammation. Primary human synovial fibroblasts cells were used to assess infection by ONNV and the resulting cytokine responses. Phenolics (gallic acid, caffeic acid and chlorogenic acid, curcumin and quercetin) and a curcuminoids-rich extract from turmeric were tested for their antiviral and anti-inflammatory capacities. We showed that infection occurred in HSF cells and increased gene expression and protein secretion of two major proinflammatory CCL-2 and IL-1β markers. In ONNV-infected HSF cells (MOI 1), we found that non-cytotoxic concentrations of phenolics (10 µM) reduced the level of viral RNA (E1, E2, nsP1, nsP2) and downregulated CCL-2 and IL-1β expression and secretion. These results highlighted the high value of the flavonol quercetin to reduce viral RNA levels and inflammatory status induced by ONNV in HSF cells.
Fichier principal
Vignette du fichier
A Malaterre Septembre Quercetin Sci reports s41598-021-85840-z.pdf (1.79 Mo) Télécharger le fichier
Origin Publication funded by an institution

Dates and versions

hal-03321865 , version 1 (18-08-2021)

Licence

Identifiers

Cite

Axelle Septembre-Malaterre, Yosra Bedoui, Claude Giry, Philippe Gasque, Pascale Guiraud, et al.. Quercetin can reduce viral RNA level of O’nyong-nyong virus and resulting innate immune cytokine responses in cultured human synovial fibroblasts. Scientific Reports, 2021, 11, pp.6369. ⟨10.1038/s41598-021-85840-z⟩. ⟨hal-03321865⟩
85 View
58 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More