HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Protective Effects of Medicinal Plant Decoctions on Macrophages in the Context of Atherosclerosis

Abstract : Atherosclerosis is a hallmark of most cardiovascular diseases. The implication of macrophages in this pathology is widely documented, notably for their contribution to lipid accumulation within the arterial wall, associated with oxidative stress and inflammation processes. In order to prevent or limit the atherosclerosis damage, nutritional approaches and medicinal plant-based therapies need to be considered. In Reunion Island, medicinal plant-based beverages are traditionally used for their antioxidant, lipid-lowering and anti-inflammatory properties. The aim of our study was to assess the protective effects of eight medicinal plant decoctions in an in vitro model of RAW 264.7 murine macrophages exposed to pro-atherogenic conditions (oxidized low-density lipoproteins—ox-LDL—E. coli Lipopolysaccharides—LPS). The impact of polyphenol-rich medicinal plant decoctions on cell viability was evaluated by Neutral Red assay. Fluorescent ox-LDL uptake was assessed by flow cytometry and confocal microscopy. Activation of NF-κB was evaluated by quantification of secreted alkaline phosphatase in RAW-Blue™ macrophages. Our results show that medicinal plant decoctions limited the cytotoxicity induced by ox-LDL on macrophages. Flow cytometry analysis in macrophages demonstrated that medicinal plant decoctions from S. cumini and P. mauritianum decreased ox-LDL uptake and accumulation by more than 70%. In addition, medicinal plant decoctions also inhibited NF-κB pathway activation in the presence of pro-inflammatory concentrations of E. coli LPS. Our data suggest that medicinal plant decoctions exert protective effects on ox-LDL-induced cytotoxicity and limited macrophage lipid uptake. Moreover, herbal preparations displayed anti-inflammatory properties on macrophages that can be of interest for limiting the atherosclerotic process
Document type :
Journal articles
Complete list of metadata

Contributor : Réunion Univ Connect in order to contact the contributor
Submitted on : Wednesday, February 3, 2021 - 7:43:07 AM
Last modification on : Friday, April 1, 2022 - 3:50:14 AM
Long-term archiving on: : Tuesday, May 4, 2021 - 6:15:40 PM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License




Eloïse Checkouri, Stéphane Ramin-Mangata, Nicolas Diotel, Wildriss Viranaicken, Claude Marodon, et al.. Protective Effects of Medicinal Plant Decoctions on Macrophages in the Context of Atherosclerosis. Nutrients, MDPI, 2021, 13 (1), pp.280. ⟨10.3390/nu13010280⟩. ⟨hal-03129664⟩



Record views


Files downloads