Skip to Main content Skip to Navigation
Journal articles

Phenolic Profile of Herbal Infusion and Polyphenol-Rich Extract from Leaves of the Medicinal Plant Antirhea borbonica: Toxicity Assay Determination in Zebrafish Embryos and Larvae

Abstract : Antirhea borbonica (A. borbonica) is an endemic plant from the Mascarene archipelago in the Indian Ocean commonly used in traditional medicine for its health benefits. This study aims (1) at exploring polyphenols profiles from two types of extracts-aqueous (herbal infusion) and acetonic (polyphenol rich) extracts from A. borbonica leaves-and (2) at evaluating their potential toxicity in vivo for the first time. We first demonstrated that, whatever type of extraction is used, both extracts displayed significant antioxidant properties and acid phenolic and flavonoid contents. By using selective liquid chromatography-tandem mass spectrometry, we performed polyphenol identification and quantification. Among the 19 identified polyphenols, we reported that the main ones were caffeic acid derivatives and quercetin-3-O-rutinoside. Then, we performed a Fish Embryo Acute Toxicity test to assess the toxicity of both extracts following the Organisation for Economic Cooperation and Development (OECD) guidelines. In both zebrafish embryos and larvae, the polyphenols-rich extract obtained by acetonic extraction followed by evaporation and resuspension in water exhibits a higher toxic effect with a median lethal concentration (LC 50 : 5.6 g/L) compared to the aqueous extract (LC 50 : 20.3 g/L). Our data also reveal that at non-lethal concentrations of 2.3 and 7.2 g/L for the polyphenol-rich extract and herbal infusion, respectively, morphological malformations such as spinal curvature, pericardial edema, and developmental delay may occur. In conclusion, our study strongly suggests that the evaluation of the toxicity of medicinal plants should be systematically carried out and considered when studying therapeutic effects on living organisms.
Document type :
Journal articles
Complete list of metadata

https://hal.univ-reunion.fr/hal-03053457
Contributor : Réunion Univ <>
Submitted on : Friday, December 11, 2020 - 9:10:15 AM
Last modification on : Friday, December 18, 2020 - 11:44:12 AM
Long-term archiving on: : Friday, March 12, 2021 - 6:51:12 PM

File

molecules-25-04482-v2.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Bryan Veeren, Batoul Ghaddar, Matthieu Bringart, Shaymaa Khazaal, Marie-Paule Gonthier, et al.. Phenolic Profile of Herbal Infusion and Polyphenol-Rich Extract from Leaves of the Medicinal Plant Antirhea borbonica: Toxicity Assay Determination in Zebrafish Embryos and Larvae. Molecules, MDPI, 2020, Phenolic Compounds from Plants: Chemistry, Analysis and Biological Activity, 25 (19), pp.4482. ⟨10.3390/molecules25194482⟩. ⟨hal-03053457⟩

Share

Metrics

Record views

55

Files downloads

37