Increased levels of soluble forms of E-selectin and ICAM-1 adhesion molecules during human leptospirosis

Abstract : Leptospirosis is a multisystemic zoonotic disease with infiltration of visceral organs by Lep-tospira. The capacity of the vascular endothelium to grant immune cell recruitment and activation in target organs during the disease course remains poorly characterized. We ascertained the levels of expression of several soluble cell adhesion molecules (CAM) notably expressed by endothelial cells in human leptospirosis. We prospectively enrolled 20 hospitalized patients and compared them to 10 healthy controls. Disease severity was defined by one or more organ failures, or death. Plasmatic concentrations of soluble CAM were assessed by multiplex bead assay at the time of patient presentation (M0) and 1 month after hospital discharge. The levels of soluble E-selectin (sCD62E) and soluble intercellular adhesion molecule 1 (sICAM-1, sCD53) were significantly increased in patients compared to controls (p<0.0001) and at 1 month (p<0.0001) with median values at 978 ng/ml (interquartile ranges 787–1164; sCD62E) and 1021 ng/ml (690–1428; sCD53). At M0, Soluble P-selectin level (sCD62P) was found to be decreased with levels at 60 ng/ml (0–631) versus 711 ng/ml (343–1113) for healthy controls (p<0.05). Levels of sICAM-3 (sCD50), sVCAM-1 (vascular cell adhesion molecule, sCD106) and sPECAM-1 (platelet endothelial cell adhesion molecule , sCD31) were not different from healthy subjects at M0. This study shows that two adhesion molecules, shed as soluble forms, are elevated during the acute phase of leptospi-rosis: E-selectin and s-ICAM1. These molecules may interfere with the process of immune cell recruitment to clear Leptospira at tissue levels.
Complete list of metadatas

Cited literature [33 references]  Display  Hide  Download

http://hal.univ-reunion.fr/hal-01702849
Contributor : Réunion Univ <>
Submitted on : Tuesday, July 3, 2018 - 1:36:53 PM
Last modification on : Thursday, July 11, 2019 - 2:10:08 PM
Long-term archiving on : Monday, October 1, 2018 - 8:43:42 AM

File

Increased_levels_of_soluble_fo...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Loïc Raffray, Claude Giry, Yoga Thirapathi, Anne-Hélène Reboux, Marie-Christine Jaffar-Bandjee, et al.. Increased levels of soluble forms of E-selectin and ICAM-1 adhesion molecules during human leptospirosis. PLoS ONE, Public Library of Science, 2017, 12 (7), pp.e0180474. ⟨10.1371/journal.pone.0180474⟩. ⟨hal-01702849⟩

Share

Metrics

Record views

171

Files downloads

42