Development of microsatellite markers in cultivated Vanilla : Polymorphism and transferability to other Vanilla species - Université de La Réunion Access content directly
Journal Articles Scientia Horticulturae Year : 2008

Development of microsatellite markers in cultivated Vanilla : Polymorphism and transferability to other Vanilla species

Abstract

The sources of natural vanilla are the cured fruits of two obligatorily hand-pollinated and clonally propagated orchids: ¿Bourbon/Mexican \textitvanilla¿ (\textitVanilla planifolia G. Jackson) and ¿\textitTahitian vanilla¿ (\textitVanilla tahitensis J.W. Moore). In this paper we describe for the first time the isolation and characterization of 14 microsatellite loci from \textitV. planifolia. These were monomorphic within cultivated accessions, as expected from the probable single clonal origin of this crop and previous genetic studies. These markers were transferable to \textitV. tahitensis and 11 loci were polymorphic between these two closely related species. Furthermore, some of these markers were transferable and polymorphic across 15 other wild American, African and Asian species and revealed consistent relationships between species, together with a strong pattern of Old World versus New World differentiation in the genus. These microsatellites will be very useful for diversity, hybridization and phylogeographic studies in the genus \textitVanilla. (Résumé d'auteur)

Domains

Vegetal Biology

Dates and versions

hal-01199371 , version 1 (15-09-2015)

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Cite

Séverine Bory, Denis da Silva, Ange-Marie Risterucci, Michel Grisoni, Pascale Besse, et al.. Development of microsatellite markers in cultivated Vanilla : Polymorphism and transferability to other Vanilla species. Scientia Horticulturae, 2008, 115 (4), pp.420--425. ⟨10.1016/j.scienta.2007.10.020⟩. ⟨hal-01199371⟩
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