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Journal Articles Journal of Agricultural and Food Chemistry Year : 2009

Metabolic Changes in Different Developmental Stages of Vanilla planifolia Pods

Abstract

The metabolomic anal. of developing Vanilla planifolia green pods (between 3 and 8 mo after pollination) was carried out by NMR spectroscopy and multivariate data anal. Multivariate data anal. of the 1H NMR spectra, such as principal component anal. (PCA) and partial least-squares-discriminant anal. (PLS-DA), showed a trend of sepn. of those samples based on the metabolites present in the methanol/water (1:1) ext. Older pods had a higher content of glucovanillin, vanillin, p-hydroxybenzaldehyde glucoside, p-hydroxybenzaldehyde, and sucrose, while younger pods had more bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-isopropyltartrate (glucoside A), bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-(2-butyl)tartrate (glucoside B), glucose, malic acid, and homocitric acid. A liq. chromatog.-mass spectrometry (LC-MS) anal. targeted at phenolic compd. content was also performed on the developing pods and confirmed the NMR results. Ratios of aglycons/glucosides were estd. and thus allowed for detection of more minor metabolites in the green vanilla pods. Quantification of compds. based on both LC-MS and NMR analyses showed that free vanillin can reach 24% of the total vanillin content after 8 mo of development in the vanilla green pods. [on SciFinder(R)]

Dates and versions

hal-01188147 , version 1 (28-08-2015)

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Tony Lionel Palama, Alfi Khatib, Young Hae Choi, Bertrand Payet, Isabelle Fock-Bastide, et al.. Metabolic Changes in Different Developmental Stages of Vanilla planifolia Pods. Journal of Agricultural and Food Chemistry, 2009, 57 (17), pp.7651--7658. ⟨10.1021/jf901508f⟩. ⟨hal-01188147⟩
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