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Journal articles
Highly Repeatable Dissolution Dynamic Nuclear Polarization for Heteronuclear NMR Metabolomics
Abstract : At natural C-13 abundance, metabolomics based on heteronuclear NMR is limited by sensitivity. We have recently demonstrated how hyperpolarization by dissolution dynamic nuclear polarization (D-DNP) assisted by cross-polarization (CP) provides a reliable way of enhancing the sensitivity of heteronudear NMR in dilute mixtures of metabolites. In this Technical Note, we evaluate the precision of this experimental approach, a critical point for applications to metabolomics. The higher the repeatability, the greater the likelihood that one can detect small biologically relevant differences between samples. The average repeatability of our state-of-the-art D-DNP NMR equipment for samples of metabolomic relevance (20 mg dry weight tomato extracts) is 3.6% for signals above the limit of quantification (LOQ) and 6.4% when all the signals above the limit of detection (LOD) are taken into account. This first report on the repeatability of D-DNP highlights the compatibility of the technique with the requirements of metabolomics and confirms its potential as an analytical tool for such applications.
https://hal.archives-ouvertes.fr/hal-01366193
Contributor : Gestionnaire Hal-Upmc <>
Submitted on : Thursday, January 21, 2021 - 2:53:32 PM
Last modification on : Friday, February 12, 2021 - 11:30:13 AM
Contributor : Gestionnaire Hal-Upmc <>
Submitted on : Thursday, January 21, 2021 - 2:53:32 PM
Last modification on : Friday, February 12, 2021 - 11:30:13 AM
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Bornet_AC_2016.pdf
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