Global analysis of VHHs framework regions with a structural alphabet: VHH FRs structures

Abstract : The VHHs are antigen-binding region/domain of camelid heavy chain antibodies (HCAb). They have many interesting biotechnological and biomedical properties due to their small size, high solubility and stability, and high affinity and specificity for their antigens. HCAb and classical IgGs are evolutionary related and share a common fold. VHHs are composed of regions considered as constant, called the frameworks (FRs) connected by Complementarity Determining Regions (CDRs), a highly variable region that provide interaction with the epitope. Actually, no systematic structural analyses had been performed on VHH structures despite a significant number of structures. This work is the first study to analyse the structural diversity of FRs of VHHs. Using a structural alphabet that allows approximating the local conformation, we show that each of the four FRs do not have a unique structure but exhibit many structural variant patterns. Moreover, no direct simple link between the local conformational change and amino acid composition can be detected. These results indicate that long-range interactions affect the local conformation of FRs and impact the building of structural models.
Liste complète des métadonnées

Littérature citée [84 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01374633
Contributeur : Alexandre De Brevern <>
Soumis le : mercredi 5 octobre 2016 - 11:07:59
Dernière modification le : dimanche 5 novembre 2017 - 15:58:07
Document(s) archivé(s) le : vendredi 6 janvier 2017 - 12:12:10

Fichier

preprint_Noel_2006_Biochimie.p...
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Floriane Noël, Alain Malpertuy, Alexandre De Brevern. Global analysis of VHHs framework regions with a structural alphabet: VHH FRs structures. Biochimie, Elsevier, 2016, 131, pp.11 - 19. 〈10.1016/j.biochi.2016.09.005〉. 〈inserm-01374633〉

Partager

Métriques

Consultations de la notice

403

Téléchargements de fichiers

47