Comparative analysis of viral RNA signatures on different RIG-I-like receptors.

Abstract : The RIG-I-like receptors (RLRs) play a major role in sensing RNA virus infection to initiate and modulate antiviral immunity. They interact with particular viral RNAs, most of them being still unknown. To decipher the viral RNA signature on RLRs during viral infection, we tagged RLRs (RIG-I, MDA5, LGP2) and applied tagged protein affinity purification followed by next-generation sequencing (NGS) of associated RNA molecules. Two viruses with negative- and positive-sense RNA genome were used: measles (MV) and chikungunya (CHIKV). NGS analysis revealed that distinct regions of MV genome were specifically recognized by distinct RLRs: RIG-I recognized defective interfering genomes, whereas MDA5 and LGP2 specifically bound MV nucleoprotein-coding region. During CHIKV infection, RIG-I associated specifically to the 3' untranslated region of viral genome. This study provides the first comparative view of the viral RNA ligands for RIG-I, MDA5 and LGP2 in the presence of infection.
Liste complète des métadonnées
Contributeur : Réunion Univ <>
Soumis le : jeudi 28 juin 2018 - 11:12:34
Dernière modification le : lundi 7 janvier 2019 - 18:00:03
Document(s) archivé(s) le : jeudi 27 septembre 2018 - 08:19:43


Fichiers éditeurs autorisés sur une archive ouverte


Distributed under a Creative Commons Paternité 4.0 International License




Raul Y Sanchez David, Chantal Combredet, Odile Sismeiro, Marie Dillies, Bernd Jagla, et al.. Comparative analysis of viral RNA signatures on different RIG-I-like receptors.. eLife, eLife Sciences Publication, 2016, 5, pp.e11275. 〈〉. 〈10.7554/eLife.11275〉. 〈hal-01483406〉



Consultations de la notice


Téléchargements de fichiers