We investigated the structure of the upper mantle beneath southeastern Brazil using teleseismic shear wave splitting measurements. Measurements were performed on seismic data recorded in the Ribeira and Brasilia Neoproterozoic belts, which wrap around the southern termination of the São Francisco craton and disappear westward under the Paraná basin. In the northern Ribeira belt, dominated by thrust tectonics, the fast shear wave polarization planes trend on average N080°E, whereas in the central domain, dominated by strike-slip tectonics, fast shear waves are polarized parallel to the structural trend (N065°E). Stations located above the main transcurrent fault display large delay times (> 2.5 s). Such values, among the largest in the world, require either an unusually large intrinsic anisotropy frozen within the lithosphere, or a contribution from both the lithospheric and asthenospheric mantle. Within the southern Brasilia belt, fast split shear waves are polarized parallel to the structural trend of the belt, at a high angle from the APM. Although part of our data set strongly favors an origin of anisotropy related to a fabric frozen in the lithospheric mantle since the Neoproterozoic, a contribution of the asthenospheric flow related to the present day plate motion is also required to explain the observed splitting parameters.