Metabolic Changes in Different Developmental Stages of Vanilla planifolia Pods

Abstract : The metabolomic anal. of developing Vanilla planifolia green pods (between 3 and 8 mo after pollination) was carried out by NMR spectroscopy and multivariate data anal. Multivariate data anal. of the 1H NMR spectra, such as principal component anal. (PCA) and partial least-squares-discriminant anal. (PLS-DA), showed a trend of sepn. of those samples based on the metabolites present in the methanol/water (1:1) ext. Older pods had a higher content of glucovanillin, vanillin, p-hydroxybenzaldehyde glucoside, p-hydroxybenzaldehyde, and sucrose, while younger pods had more bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-isopropyltartrate (glucoside A), bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-(2-butyl)tartrate (glucoside B), glucose, malic acid, and homocitric acid. A liq. chromatog.-mass spectrometry (LC-MS) anal. targeted at phenolic compd. content was also performed on the developing pods and confirmed the NMR results. Ratios of aglycons/glucosides were estd. and thus allowed for detection of more minor metabolites in the green vanilla pods. Quantification of compds. based on both LC-MS and NMR analyses showed that free vanillin can reach 24% of the total vanillin content after 8 mo of development in the vanilla green pods. [on SciFinder(R)]
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Article dans une revue
Journal of Agricultural and Food Chemistry, American Chemical Society, 2009, 57 (17), pp.7651--7658. 〈10.1021/jf901508f〉
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http://hal.univ-reunion.fr/hal-01188147
Contributeur : Claire Tessier <>
Soumis le : vendredi 28 août 2015 - 15:08:11
Dernière modification le : vendredi 14 septembre 2018 - 08:16:01

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Tony Lionel Palama, Alfi Khatib, Young Hae Choi, Bertrand Payet, Isabelle Fock, et al.. Metabolic Changes in Different Developmental Stages of Vanilla planifolia Pods. Journal of Agricultural and Food Chemistry, American Chemical Society, 2009, 57 (17), pp.7651--7658. 〈10.1021/jf901508f〉. 〈hal-01188147〉

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