Evidence of parietal amine oxidase activity in Solanum torvum Sw. stem calli after Ralstonia solanacearum inoculation

Abstract : Calli induced from Solanum torvum stem explants were inoculated with Ralstonia solanacearum under partial vacuum. All calli showed a hypersensitive response after infiltration. Furthermore, amine oxidase activity with aldehyde and H2O2 prodn. was detected in semi-purified cell walls of calli infiltrated by the bacteria. Due to its preferential affinity for monoamines, this enzyme is supposed to have monoamine oxidase-like (MAO-like) activity. Moreover, the presence of hydroxyl radicals in the arom. cycle alters the oxidative deamination kinetics of potential substrates. Indeed, the oxidn. of dopamine (+2, OH) was shown to be faster than that of tyramine (+1, OH), which in turn was faster than that of phenylethylamine (0, OH). The MAO-like catalytic activity was significantly inhibited by some reducing agents such as sodium bisulfite and cysteine, and also by tryptamine under anaerobiosis. This latter result suggested that the prosthetic group of the MAO-like enzyme could be a tyrosine-derived 6-hydroxytopaquinone structure. Finally, the sigmoid kinetics of the MAO-like enzyme in semi-purified cell walls did not correspond to that expected for a purified MAO, suggesting that the kinetics were affected by some factors present in cell walls. [on SciFinder(R)]
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Plant Physiology and Biochemistry, Elsevier, 2009, 47 (4), pp.313--321. 〈10.1016/j.plaphy.2008.12.025〉
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http://hal.univ-reunion.fr/hal-01188140
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Soumis le : vendredi 28 août 2015 - 15:07:51
Dernière modification le : mercredi 7 février 2018 - 09:06:02

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Marcel Aribaud, Michel Noirot, Anne Gauvin-Bialecki, Christine Da Silva-Robert, Isabelle Fock, et al.. Evidence of parietal amine oxidase activity in Solanum torvum Sw. stem calli after Ralstonia solanacearum inoculation. Plant Physiology and Biochemistry, Elsevier, 2009, 47 (4), pp.313--321. 〈10.1016/j.plaphy.2008.12.025〉. 〈hal-01188140〉

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